Biomodel

Virtual Laboratories

Cybertory: virtual molecular biology lab

Access:

enter Enter the virtual laboratory.

enterSequence analysis - Direct link to the tools (transcription-translation, sequence pattern search, sequence massager)

página General instructions for the use of Cybertory.

Features:

Possible applications:

Workbooks:

  1A 1B 1C 1D 2 3 4 5 6
using electrophoresis sí sí sí   sí sí sí sí sí
using restriction enzymes sí sí     sí        
using PCR         sí sí sí sí sí

páginaGeneral instructions for the use of Cybertory.

  1. Genetic diagnosis; βAS polymorphism in the beta globin gene, responsible for drepanocytosis and drepanocytic anaemia (sickle cell anaemia):
    1A pdf Testing restriction enzymes for the analysis of this polymorphism by RFLP. doi:10.5281/zenodo.1299796
        mostrarSome enzyme sets suitable for this practical.
    1B pdf Molecular genetic diagnosis of the polymorphism by RFLP. doi:10.5281/zenodo.1299811
    1C pdf DNA sequencing to analyse this polymorphism (dideoxynucleotides method). doi:10.5281/zenodo.1299816
    1D pdf Introduction to genomic sequence databases and analysis of sequences related to this polymorphism. doi:10.5281/zenodo.1299825
  2. Forensic analysis: genetic identification of individuals:
    2A pdf Using RFLP. doi:10.5281/zenodo.1299843
    2B pdf Using multiplex PCR on STR markers (CoDIS). doi:10.5281/zenodo.3607339
  3. pdf Paternity test using multiplex PCR on STR markers (CoDIS). https://doi.org/10.5281/zenodo.1299837
  4. pdf Genetic diagnosis, personalised medicine. Identification of polymorphic variants on cytochrome P450 (CYP2C9), using multiplex PCR. doi:10.5281/zenodo.1299846
  5. pdf Genetic diagnosis of celiac disease. Identification of polymorphic variant associated to celaic disease, using multiplex PCR on the DQ2 and DQ8 markers. doi:10.5281/zenodo.1299854
  6. pdf Dairy product analysis. Identification of the source of milk (cow, sheep or goat) by analysis of mitochondrial DNA. doi:10.5281/zenodo.1299860

Simulation of celulose acetate electrophoresis of proteins

Features:

Possible applications:

Access:

Access the simulator entrada for serum proteins and entrada for LDH isoenzymes.

Simulation of SDS-PAGE electrophoresis of proteins

Features:

Possible applications:

Workbooks:

These are integrated into the simulation page:

mostrarPractice problem 1: influence of the voltage applied.

mostrarPractice problem 2: influence of the acrylamide concentration.

mostrarPractice problem 3: estimation of molecular mass of the unknown proteins.

mostrarPractice problem 4: determination of quaternary structure (I).

mostrarPractice problem 5: determination of quaternary structure (II).

Access:

entrada Acces the simulator.

UV-VIS spectrophotometry laboratories

Simulator of ultraviolet absorption spectra for proteins and nucleic acids

Access:

enter Access the simulator.

Features:

  • Protein and DNA absorption spectrum between 230 and 340 nm.
  • Absorbance values at 260 and 280 nm, and the ratio between both.
  • Predefined profiles: proteins, DNA and four mixtures.
  • Custom profiles based on proportion provided for each type of molecule.
  • Possible to superimpose sucessive spectra to compare them.

Applications:

  • Demonstration of the analytical value of the technique for checking DNA contamination with proteins or vice versa.

Virtual UV-VIS spectrophotometer

Access:

enter Access the virtual laboratory.roadworks

Features:

  • Absroption spectrum of diverse substances between 200 and 800 nm.
  • Preparation of dilutions.

Applications:

  • Relation between absorbance and concentration.
  • Fundamentals of a protein quantitation assay.
  • Determination of absorption spectra; search for λmax

Laboratories with the roadworks icon are not finished and may fail. Suggestions are welcome.

Colorimetric assay for enzyme activity

Access:

enter Access the virtual laboratory.

Features:

  • Enzyme activity assay.
  • Effect of pH and temperature on the activity of an enzyme.

Applications:

  • Search for optimal conditions for the enzyme.

Simulation of column cromatography of proteins

Features:

Possible applications:

Access:

entrada Access the simulator.

Working plans:

Project: design of problem-solving activities using the simulator.

Other labs

These are ready but not yet translated into English. You can check the Spanish versions or, if you are interested on them, for setting an English translation.