Enzymes

Influence of substrate concentration

Graphical analysis of Michaelis & Menten kinetics.

Graphical interpretation of parameters K_m and V_max

Calculations

Fitting experimental data to Michaelis & Menten equation using nonlinear regression.

Linearised plots.

Comparison of graphical plots: direct (Michaelis & Menten), double reciprocals (Lineweaver & Burk), Eadie & Hofstee, Hanes & Woolf.

Influence of enzyme concentration

Influence of enzyme concentration; k_cat parameter

Cooperativity

Cooperativity; Graphical interpretation of Hill's coefficient.