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Enzymes

Enzyme kinetics

Influence of substrate concentration

Graphical analysis of Michaelis & Menten kinetics.

Graphical interpretation of parameters Km and Vmax

Calculations

Fitting experimental data to Michaelis & Menten equation using nonlinear regression.

Linearised plots.

Comparison of graphical plots: direct (Michaelis & Menten), double reciprocals (Lineweaver & Burk), Eadie & Hofstee, Hanes & Woolf.

 

Influence of enzyme concentration

Influence of enzyme concentration; kcat parameter

Cooperativity

Cooperativity; Graphical interpretation of Hill's coefficient.

Disponible también en español.