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Enzyme Kinetics

Michaelis - Menten model - Linear plots

Direct plot (Michaelis - Menten)   Double reciprocal plot (Lineweaver - Burk)  
v
[S]

Type some values:

Km = mM

Vmax = µM min−1

N. points:
Replicates:
Experimental error: %

1/v
1/[S]

Result of regression:

Km = ... mM

Vmax = ... µM min−1

Deviation:
... in log(Km)
... in Vmax

Hanes - Woolf plot   Eadie - Hofstee plot  
[S]/v
[S]

Result of regression:

Km = ... mM

Vmax = ... µM min−1

Deviation:
... in log(Km)
... in Vmax

v
v/[S]

Result of regression:

Km = ... mM

Vmax = ... µM min−1

Deviation:
... in log(Km)
... in Vmax