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Enzyme Kinetics

Michaelis - Menten model - Linear plots

Direct plot (Michaelis - Menten)

Double reciprocal plot (Lineweaver - Burk)

v

[S]

Type some values:

K_m = mM

V_max = µM min⁻¹

N. points:
Replicates:
Experimental error: %

1/v

1/[S]

Result of regression:

K_m = ... mM

V_max = ... µM min⁻¹

Deviation:
... in log(K_m)
... in V_max

Hanes - Woolf plot

Eadie - Hofstee plot

[S]/v

[S]

Result of regression:

K_m = ... mM

V_max = ... µM min⁻¹

Deviation:
... in log(K_m)
... in V_max

v

v/[S]

Result of regression:

K_m = ... mM

V_max = ... µM min⁻¹

Deviation:
... in log(K_m)
... in V_max