SIMENKIN, Module 1

This first module uses linear regression to obtain the slope and intercept of the Lineweaver–Burk plot for a given set of data.

This module is designed to operationalise the kinetic characterisation of an enzyme. The user is confronted with the task of translating the raw experimental data of initial rate and substrate concentration into kinetic parameters.

This module allows the input of substrate concentration and initial velocity data taken from an enzyme-catalysed assay. The plot slope and the intercepts at the two axes provide information about the kinetic parameters of the enzyme.

This module shows how, in spite of some unavoidable experimental errors, Km and ѵmax can be determined and how these parameters relate to the experimental conditions. Since it gives an almost instant output from the data input, it provides a direct impression of the impact of the experimental measurements on the kinetic parameters.

Input data:

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1/[S] 1/ѵ

Output:

Slope:
Y-intercept:
X-intercept:

Proceed to module 2 >>