PCR Titration Calculator

Experiment title:
Investigator: Date:
Reaction volume: µl Fudge factor*: %
Titrate Background Upper Both Reagent Initial concentration Final concentration (the highest in the titration)
buffer x x
4 dNTP µM each µM each
MgCl2 mM mM
5' primer µM µM
3' primer µM µM

red sucrose

x x
template x x
polymerase U/µl U/µl
additive x x
(*) fudge factor
A. noun
    1. correction, fudge factor
               a quantity that is added or subtracted in order to increase the accuracy of a scientific measure.