Practice activities
to learn using the cellulose acetate electrophoresis simulator

The purpose of these activities or exercises is that you learn by discovery, using the simulator to find out the answer. Advice: open this page and the simulator in separate windows and jump from one to the other while you solve the questions.

Serum proteins

1. ¿Which is the slowest protein in electrophoresis?
   
2. ¿Which is the protein with the lowest isoelectric point?
   
3. ¿What happens if the volume of sampple applied is increased?
   Bands separate... better worse similarly
4. and, at the same time,...
   Band intensity and peak height in the densitogram... are higher are lower do not change
5. ¿In which pathological situation gammaglobulins experience a stronger increase?
   nephrotic syndrome ferropenic anaemia hepatic cirrhosis infection
6. ¿Which protein fraction is more increased in infection?
   
7. In stress response, when going from early to late stage, ¿which protein fraction rises?
   
8. and ¿which protein fraction diminishes?
   
9. In starving there is a general reduction in all proteins. By adjusting the volume of sample applied to the acetate strip, find out whether any protein fraction is more affected than the others, or else all of them are equally reduced.
   all the same albumin is diminished less than the others albumin is diminished more than the others β globulins are diminished more than the others α1 and α2 globulins are diminished more than the others
10. A patient yields the following test results: 72 g/ℓ total protein, 53% albumin, 2,4% α1, 9,1% α2, 24% β, 11,4% γ. ¿Which could be his/her state?
    healthy infection cirrhosis ferropenic anaemia paraprotein stress nephrotic syndrome

your answers.

Angel Herráez. Part of the Biomodel.uah.es website