Enzyme Kinetics

Type some values:

K_m = mM

V_max = µM min⁻¹

Activities

1. If K_m has a 5 mM value, how much substrate is needed to reach 90% of maximum velocity?
   4 mM 5 mM 50 mM 150 mM
2. If K_m has a 5 mM value, how much substrate is needed to reach 90% of maximum velocity?
   1.5 mM 2 mM 6 mM 20 mM
3. For a given substrate concentration, an increase in K_m may be interpreted as
   an increase a decrease
   in enzyme efficiency
4. Two isoenzymes have K_m of 3 mM and 30 mM. Which one will be more sensitive to fluctuations in substrate between 20 and 30 mM?
   the one with lower K_m the one with higher K_m