PCR Calculator

PCR Titration Calculator

Experiment title:
Investigator:						Date:
	Columns	Rows		Reaction volume: µl		Fudge factor*: %
Titrate	Background	Upper	Both	Reagent	Initial concentration	Final concentration (the highest in the titration)
				buffer	x	x
				4 dNTP	µM each	µM each
				MgCl₂	mM	mM
				5' primer	µM	µM
				3' primer	µM	µM
				red sucrose	x	x
				template	x	x
				polymerase	U/µl	U/µl
				additive	x	x
				H₂O

(*) fudge factor
A. noun
1. correction, fudge factor
a quantity that is added or subtracted in order to increase the accuracy of a scientific measure.